| # | English Conference | G | W | D | L | GF | GA | Pts | +- | ||
| ^ | Stevenage Borough | 44 | 30 | 9 | 5 | 79 | 24 | 99 | 55 | Promoted | |
| - | Luton Town | 44 | 26 | 10 | 8 | 84 | 40 | 88 | 44 | ||
| ^ | Oxford United | 44 | 25 | 11 | 8 | 64 | 31 | 86 | 33 | Promoted | |
| - | Rushden and Diamonds | 44 | 22 | 13 | 9 | 77 | 39 | 79 | 38 | ||
| - | York City | 44 | 22 | 12 | 10 | 62 | 35 | 78 | 27 | ||
| - | Kettering Town | 44 | 18 | 12 | 14 | 51 | 41 | 66 | 10 | ||
| - | Crawley Town | 44 | 19 | 9 | 16 | 50 | 57 | 66 | -7 | ||
| - | AFC Wimbledon | 44 | 18 | 10 | 16 | 61 | 47 | 64 | 14 | ||
| - | Mansfield Town | 44 | 17 | 11 | 16 | 69 | 60 | 62 | 9 | ||
| - | Cambridge United | 44 | 15 | 14 | 15 | 65 | 53 | 59 | 12 | ||
| - | Wrexham | 44 | 15 | 13 | 16 | 45 | 39 | 58 | 6 | ||
| * | Salisbury City* | 44 | 21 | 5 | 18 | 58 | 63 | 58 | -5 | Relegated | |
| - | Kidderminster Harriers | 44 | 15 | 12 | 17 | 57 | 52 | 57 | 5 | ||
| - | Altrincham | 44 | 13 | 15 | 16 | 53 | 51 | 54 | 2 | ||
| - | Barrow | 44 | 13 | 13 | 18 | 50 | 67 | 52 | -17 | ||
| - | Tamworth | 44 | 11 | 16 | 17 | 42 | 52 | 49 | -10 | ||
| - | Hayes and Yeading United | 44 | 12 | 12 | 20 | 59 | 85 | 48 | -26 | ||
| - | Histon | 44 | 11 | 13 | 20 | 44 | 67 | 46 | -23 | ||
| - | Eastbourne Borough | 44 | 11 | 13 | 20 | 42 | 72 | 46 | -30 | ||
| - | Gateshead* | 44 | 13 | 7 | 24 | 46 | 69 | 45 | -23 | ||
| - | Forest Green Rovers | 44 | 12 | 9 | 23 | 50 | 76 | 45 | -26 | ||
| * | Ebbsfleet United | 44 | 12 | 8 | 24 | 50 | 82 | 44 | -32 | Relegated | |
| * | Grays Athletic* | 44 | 5 | 13 | 26 | 35 | 91 | 26 | -56 | Relegated | |
| - | Chester City | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 2010-04-29 | Rushden and Diamonds Byrne 51 | 1-1 | Oxford United Constable 29 |
| York City Brodie 90 | 1-0 | Luton Town | |
| 2010-05-03 | Oxford United Matthew Green 53 James Constable 57 | 2-0 | Rushden and Diamonds |
| 2010-05-16 | Oxford United Matt Green 15\n James Constable 20\n Alfie Potter 90 | 3-1 | York City Clarke 42 (og) |